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npcs derived from xcl-1 ipscs  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc npcs derived from xcl-1 ipscs
    (A) Brightfield image of Cas9-expressing <t>NPCs</t> used for CRISPR experiments. (B) MAP2 and DAPI staining of NPCs. NPC-Cas9hy p7 was seeded as single cells in a 24-well plate pre-coated with GFR-Matrigel, at 200,000 cells per well. Cell culture medium NPC + Hygromycin (100 ug/ml) was changed every other day. Cells were fixed at d9 with 4% PFA for 15 min and stained for MAP2. (C) Gel image of T7 Endonuclease I assay result. Multiple cleavage products in the KO cells indicate genome editing at the intended site. Band intensities of full-length and cleavage products were quantified using Fiji, and gene editing efficiency was calculated using the following equation: 100 x (1 – (1-fraction cleaved)1/2) (see Methods). (D) Western blot results confirm the perturbation effects for m6A-associated RBPs. Whole-cell lysates from KO or NT control cells were subjected to western blot analysis using the Jess Simple Western platform. Digital gel images are shown for the 6 designated targets, probed for both Actin (red) and antibodies for the corresponding KO target (black). Actin-normalized intensities of KO targets are shown at the bottom.
    Npcs Derived From Xcl 1 Ipscs, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/npcs+derived+from+xcl-1+ipscs/npcs+xcl+1+line/bio_rxiv__2024__10__23__619425-230-0-11
    Average 90 stars, based on 1 article reviews
    npcs derived from xcl-1 ipscs - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease"

    Article Title: The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease

    Journal: bioRxiv

    doi: 10.1101/2024.10.23.619425

    (A) Brightfield image of Cas9-expressing NPCs used for CRISPR experiments. (B) MAP2 and DAPI staining of NPCs. NPC-Cas9hy p7 was seeded as single cells in a 24-well plate pre-coated with GFR-Matrigel, at 200,000 cells per well. Cell culture medium NPC + Hygromycin (100 ug/ml) was changed every other day. Cells were fixed at d9 with 4% PFA for 15 min and stained for MAP2. (C) Gel image of T7 Endonuclease I assay result. Multiple cleavage products in the KO cells indicate genome editing at the intended site. Band intensities of full-length and cleavage products were quantified using Fiji, and gene editing efficiency was calculated using the following equation: 100 x (1 – (1-fraction cleaved)1/2) (see Methods). (D) Western blot results confirm the perturbation effects for m6A-associated RBPs. Whole-cell lysates from KO or NT control cells were subjected to western blot analysis using the Jess Simple Western platform. Digital gel images are shown for the 6 designated targets, probed for both Actin (red) and antibodies for the corresponding KO target (black). Actin-normalized intensities of KO targets are shown at the bottom.
    Figure Legend Snippet: (A) Brightfield image of Cas9-expressing NPCs used for CRISPR experiments. (B) MAP2 and DAPI staining of NPCs. NPC-Cas9hy p7 was seeded as single cells in a 24-well plate pre-coated with GFR-Matrigel, at 200,000 cells per well. Cell culture medium NPC + Hygromycin (100 ug/ml) was changed every other day. Cells were fixed at d9 with 4% PFA for 15 min and stained for MAP2. (C) Gel image of T7 Endonuclease I assay result. Multiple cleavage products in the KO cells indicate genome editing at the intended site. Band intensities of full-length and cleavage products were quantified using Fiji, and gene editing efficiency was calculated using the following equation: 100 x (1 – (1-fraction cleaved)1/2) (see Methods). (D) Western blot results confirm the perturbation effects for m6A-associated RBPs. Whole-cell lysates from KO or NT control cells were subjected to western blot analysis using the Jess Simple Western platform. Digital gel images are shown for the 6 designated targets, probed for both Actin (red) and antibodies for the corresponding KO target (black). Actin-normalized intensities of KO targets are shown at the bottom.

    Techniques Used: Expressing, CRISPR, Staining, Cell Culture, T7EI Assay, Western Blot, Control, Simple Western

    Related Articles

    Derivative Assay:

    Article Title: The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease
    Article Snippet: Neural progenitor cells (NPCs) derived from XCL-1 iPSCs were obtained from Stem Cell Technologies.

    Expressing:

    Article Title: The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease
    Article Snippet: Neural progenitor cells (NPCs) derived from XCL-1 iPSCs were obtained from Stem Cell Technologies.

    CRISPR:

    Article Title: The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease
    Article Snippet: Neural progenitor cells (NPCs) derived from XCL-1 iPSCs were obtained from Stem Cell Technologies.

    Staining:

    Article Title: The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease
    Article Snippet: Neural progenitor cells (NPCs) derived from XCL-1 iPSCs were obtained from Stem Cell Technologies.

    Cell Culture:

    Article Title: The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease
    Article Snippet: Neural progenitor cells (NPCs) derived from XCL-1 iPSCs were obtained from Stem Cell Technologies.

    T7EI Assay:

    Article Title: The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease
    Article Snippet: Neural progenitor cells (NPCs) derived from XCL-1 iPSCs were obtained from Stem Cell Technologies.

    Western Blot:

    Article Title: The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease
    Article Snippet: Neural progenitor cells (NPCs) derived from XCL-1 iPSCs were obtained from Stem Cell Technologies.

    Control:

    Article Title: The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease
    Article Snippet: Neural progenitor cells (NPCs) derived from XCL-1 iPSCs were obtained from Stem Cell Technologies.

    Simple Western:

    Article Title: The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease
    Article Snippet: Neural progenitor cells (NPCs) derived from XCL-1 iPSCs were obtained from Stem Cell Technologies.



    Similar Products

    npcs derived from xcl-1 ipscs  (STEMCELL Technologies Inc)
    90
    STEMCELL Technologies Inc npcs derived from xcl-1 ipscs
    (A) Brightfield image of Cas9-expressing <t>NPCs</t> used for CRISPR experiments. (B) MAP2 and DAPI staining of NPCs. NPC-Cas9hy p7 was seeded as single cells in a 24-well plate pre-coated with GFR-Matrigel, at 200,000 cells per well. Cell culture medium NPC + Hygromycin (100 ug/ml) was changed every other day. Cells were fixed at d9 with 4% PFA for 15 min and stained for MAP2. (C) Gel image of T7 Endonuclease I assay result. Multiple cleavage products in the KO cells indicate genome editing at the intended site. Band intensities of full-length and cleavage products were quantified using Fiji, and gene editing efficiency was calculated using the following equation: 100 x (1 – (1-fraction cleaved)1/2) (see Methods). (D) Western blot results confirm the perturbation effects for m6A-associated RBPs. Whole-cell lysates from KO or NT control cells were subjected to western blot analysis using the Jess Simple Western platform. Digital gel images are shown for the 6 designated targets, probed for both Actin (red) and antibodies for the corresponding KO target (black). Actin-normalized intensities of KO targets are shown at the bottom.
    Npcs Derived From Xcl 1 Ipscs, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/npcs+derived+from+xcl-1+ipscs/npcs+xcl+1+line/bio_rxiv__2024__10__23__619425-230-0-11
    Average 90 stars, based on 1 article reviews
    npcs derived from xcl-1 ipscs - by Bioz Stars, 2026-11
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Brightfield image of Cas9-expressing NPCs used for CRISPR experiments. (B) MAP2 and DAPI staining of NPCs. NPC-Cas9hy p7 was seeded as single cells in a 24-well plate pre-coated with GFR-Matrigel, at 200,000 cells per well. Cell culture medium NPC + Hygromycin (100 ug/ml) was changed every other day. Cells were fixed at d9 with 4% PFA for 15 min and stained for MAP2. (C) Gel image of T7 Endonuclease I assay result. Multiple cleavage products in the KO cells indicate genome editing at the intended site. Band intensities of full-length and cleavage products were quantified using Fiji, and gene editing efficiency was calculated using the following equation: 100 x (1 – (1-fraction cleaved)1/2) (see Methods). (D) Western blot results confirm the perturbation effects for m6A-associated RBPs. Whole-cell lysates from KO or NT control cells were subjected to western blot analysis using the Jess Simple Western platform. Digital gel images are shown for the 6 designated targets, probed for both Actin (red) and antibodies for the corresponding KO target (black). Actin-normalized intensities of KO targets are shown at the bottom.

    Journal: bioRxiv

    Article Title: The YTHDF Proteins Shape the Brain Gene Signatures of Alzheimer’s Disease

    doi: 10.1101/2024.10.23.619425

    Figure Lengend Snippet: (A) Brightfield image of Cas9-expressing NPCs used for CRISPR experiments. (B) MAP2 and DAPI staining of NPCs. NPC-Cas9hy p7 was seeded as single cells in a 24-well plate pre-coated with GFR-Matrigel, at 200,000 cells per well. Cell culture medium NPC + Hygromycin (100 ug/ml) was changed every other day. Cells were fixed at d9 with 4% PFA for 15 min and stained for MAP2. (C) Gel image of T7 Endonuclease I assay result. Multiple cleavage products in the KO cells indicate genome editing at the intended site. Band intensities of full-length and cleavage products were quantified using Fiji, and gene editing efficiency was calculated using the following equation: 100 x (1 – (1-fraction cleaved)1/2) (see Methods). (D) Western blot results confirm the perturbation effects for m6A-associated RBPs. Whole-cell lysates from KO or NT control cells were subjected to western blot analysis using the Jess Simple Western platform. Digital gel images are shown for the 6 designated targets, probed for both Actin (red) and antibodies for the corresponding KO target (black). Actin-normalized intensities of KO targets are shown at the bottom.

    Article Snippet: Neural progenitor cells (NPCs) derived from XCL-1 iPSCs were obtained from Stem Cell Technologies.

    Techniques: Expressing, CRISPR, Staining, Cell Culture, T7EI Assay, Western Blot, Control, Simple Western